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Detection of hybridization by chemiluminescence was carried out as described for Southern blots. Intergenic spacing in the Staphylococcus epidermidis operon differed somewhat from those listed above. Neidhardt F C, et al. Surprisingly, however, complementation of the sloC mutant with the sloC -bearing plasmid, pVA, did not restore virulence. This may also occur with SloC, with overproduction resulting in increased extracellular secretion. This construct was introduced into the chromosome of the cariogenic isolate, V, by a Campbell-type recombination. This article has been cited by other articles in PMC. The Bam HI-containing ends of the two amplicons were complementary to one another, such that use of the two amplicons as a template in a third PCR with primers P1 and P4 resulted in amplification of a product equivalent to the ligation of the first two products at their Bam HI sites.
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The council then voted to appoint Lightner as council president, with Gloria and David Alvarez in opposition. What has the author Todd Michael written? Members of the California State Assembly.
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